Disk Diffusion Simulation
Kirby-BauerAntibiotic Susceptibility Testing · CLSI M100
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This test is standardized by CLSI (Clinical and Laboratory Standards Institute) guidelines.
Kirby-Bauer Disk Diffusion
Perform the standard CLSI antibiotic susceptibility test — used daily in clinical microbiology labs worldwide.
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9 Steps
Lab protocol
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6 Organisms
ATCC strains
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7 Antibiotics
CLSI breakpoints
Learning Objectives
- Prepare and inoculate Mueller-Hinton agar
- Place antibiotic disks following CLSI spacing guidelines
- Measure zones of inhibition accurately
- Interpret S/I/R using CLSI M100 breakpoints
- Generate a professional lab susceptibility report