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Beer‑Lambert Law Calculator
A = ε · c · l
Spectrophotometry
Calculation Method
Absorbance (A)
Concentration (c)
Molar absorptivity (ε)
Units
Concentration
M
mM
μM
nM
Path length
cm
mm
m
Molar absorptivity ε (M⁻¹cm⁻¹)
Concentration c (M)
Path length l (cm)
Absorbance A
Common Compounds
NADH
ε 6220 · 340 nm
DNA
ε 6600 · 260 nm
BSA
ε 55000 · 280 nm
Methylene Blue
ε 95000 · 665 nm
Calculate
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About Beer‑Lambert Law
Calibration Curve
Beer‑Lambert Law
A = ε · c · l
T = 10⁻ᴬ · 100%
