Chromatographic Resolution Calculator

Rₛ = 2(t₂−t₁)/(w₁+w₂)

Separation Science

Retention time 1

Retention time 2

Peak width 1

Peak width 2

Examples

Simulated Peaks

Resolution Criteria

Rₛ > 1.5: baseline separation
Rₛ = 1.0: 94% separated
Rₛ < 0.8: overlap